A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein.
Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokarfyotic expression systems.
1. Qian, Z. et al., 2001, J Biol Chem. 276 :22439-45.
2. Lin, JL. et al., 2001, Oncogene. 20: 6559-6569.
3. Sung V, et al., 2003, Cancer research. 63: 3356-63.
4. Ma, X. et al., 2007, Molecular biology of the cell. 18:1965-78.
5. ten Klooster JP, et al., 2009, Developmental cell. 16:551-62.