Human WARS / TrpRS Gene ORF cDNA clone expression plasmid,without any tag

Gene

Species

Human

NCBI Ref Seq

BC017489

RefSeq ORF Size

1416bp

Gene Synonym

IFI53, IFP53, GAMMA-2

Sequence Description

Identical with the Gene Bank Ref. ID sequence.

Description

Full length Clone DNA of Human tryptophanyl-tRNA synthetase Gene ORF cDNA clone expression plasmid,without any tag

Plasmid

Promoter

Enhanced CMV mammalian cell promoter

Vector

pCMV3-untagged

Restriction Site

Protein Tag

Tag Sequence

Sequencing Primers

Forward:T7(TAATACGACTCACTATAGGG) Reverse:BGH(TAGAAGGCACAGTCGAGG)

Quality Control

The plasmid is confirmed by full-length sequencing.

Screening

Antibiotic in E.coli

Ampicillin

Antibiotic in Mammalian cell

Hygromycin

Application

Stable or Transient mammalian expression

Storage & Shipping

Shipping

Each tube contains lyophilized plasmid.

Storage

The lyophilized plasmid can be stored at ambient temperature for three months.

Other Life Science vendor

Natural small molecules manufacturer | Biological Research Reagents Manufacturer

Background Information

WARS, also known as TrpRS, is an aminoacyl-tRNA synthetase which belongs to the class-I aminoacyl-tRNA synthetase family. There are two forms of tryptophanyl-tRNA synthetase: a cytoplasmic form, named WARS, and a mitochondrial form, named WARS2. They catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. WARS catalyzes the aminoacylation of tRNA(trp) with tryptophan and is induced by interferon.

References

Buwitt U. et al., 1992, EMBO J. 11 (2): 489-96.

Fleckner J. et al., 1992, Proc Natl Acad Sci. 88 (24): 11520-4.

Ewalt KL. et al., 2002, Biochemistry. 41 (45): 13344-9.

Catalog Number:MGI409-UT