Mouse FAM3C / ILEI Gene ORF cDNA clone expression plasmid,C terminal GFP tag

Gene

Species

Mouse

NCBI Ref Seq

NM_138587.4

RefSeq ORF Size

684bp

Gene Synonym

Ilei; D6Wsu176e

Sequence Description

Identical with the Gene Bank Ref. ID sequence.

Description

Full length Clone DNA of Mouse family with sequence similarity 3, member C Gene ORF cDNA clone expression plasmid,C terminal GFP tag

Plasmid

Promoter

Enhanced CMV mammalian cell promoter

Vector

pCMV3-C-GFPSpark

Restriction Site

Protein Tag

GFPSpark

Tag Sequence

GTGAGCAAGGGC……GAGCTGTACAAG

Sequencing Primers

Forward:T7(TAATACGACTCACTATAGGG) Reverse:BGH(TAGAAGGCACAGTCGAGG)

Quality Control

The plasmid is confirmed by full-length sequencing.

GFPSpark Tag Information

GFPSpark is an improved variant of the green fluorescent protein GFP. It possesses bright green fluorescence (excitation/ emission max = 487 / 508 nm) that is visible earlier than fluorescence of other green fluorescent proteins. GFPSpark is mainly intended for applications where fast appearance of bright fluorescence is crucial. It is specially recommended for cell and organelle labeling and tracking the promoter activity.

Screening

Antibiotic in E.coli

Kanamycin

Antibiotic in Mammalian cell

Hygromycin

Application

Stable or Transient mammalian expression

Storage & Shipping

Shipping

Each tube contains lyophilized plasmid.

Storage

The lyophilized plasmid can be stored at ambient temperature for three months.

Other Life Science vendor

Natural small molecules manufacturer | Biological Research Reagents Manufacturer

Background Information

FAM3C, also known as ILEI, is a member of the FAM3 family. It can be detected in resent in most secretory epithelia. FAM3C may be involved in retinal laminar formation. It promotes epithelial to mesenchymal transition. A change in expression of FAM3C has been noted in pancreatic cancer-derived cells. ILEI is overexpressed and/or altered in intracellular localization in multiple human tumors, an event strongly correlated to invasion/EMT, metastasis formation, and survival in human colon and breast cancer.

References

Zhu Y. et al., 2002, Genomics. 80 (2): 144-50.

Strausberg RL. et al., 2003, Proc Natl Acad Sci. 99 (26): 16899-903.

Quéméner-Redon S. et al., 2003, Nature. 424 (6945): 157-64.

Catalog Number:MGC692-CG