The plasmid is confirmed by full-length sequencing.
GFPSpark Tag Information
GFPSpark is an improved variant of the green fluorescent protein GFP. It possesses bright green fluorescence (excitation/ emission max = 487 / 508 nm) that is visible earlier than fluorescence of other green fluorescent proteins. GFPSpark is mainly intended for applications where fast appearance of bright fluorescence is crucial. It is specially recommended for cell and organelle labeling and tracking the promoter activity.
Screening
Antibiotic in E.coli
Kanamycin
Antibiotic in Mammalian cell
Hygromycin
Application
Stable or Transient mammalian expression
Storage & Shipping
Shipping
Each tube contains lyophilized plasmid.
Storage
The lyophilized plasmid can be stored at ambient temperature for three months.
This Aurora B kinase gene encodes a member of the aurora kinase subfamily of serine/threonine kinases. The Aurora B kinase genes encoding the other two members of this subfamily are located on chromosomes 19 and 20. These kinases participate in the regulation of alignment and segregation of chromosomes during mitosis and meiosis through association with microtubules. A pseudogene of this gene is located on chromosome 8. Alternatively spliced transcript variants have been found for this gene.
References
Xavier de Carvalho A, Maiato H, Maia AF, et al. Reed-Sternberg Cells Form by Abscission Failure in the Presence of Functional Aurora B Kinase. Zhang L, ed. PLoS ONE. 2015;10(5):e0124629.
Mouse Aurora B Gene ORF cDNA clone expression plasmid,C terminal GFP tag, Related Products