Human SART3 Gene ORF cDNA clone expression plasmid,without any tag

Gene

Species

Human

NCBI Ref Seq

NM_014706.3

RefSeq ORF Size

2892 bp

Gene Synonym

SART3

Sequence Description

Identical with the Gene Bank Ref. ID sequence.

Description

Full length Clone DNA of Human squamous cell carcinoma antigen recognized by T-cells 3 Gene ORF cDNA clone expression plasmid,without any tag

Plasmid

Promoter

Enhanced CMV mammalian cell promoter

Vector

pCMV3-untagged

Restriction Site

Protein Tag

Tag Sequence

Sequencing Primers

Forward:T7(TAATACGACTCACTATAGGG) Reverse:BGH(TAGAAGGCACAGTCGAGG)

Quality Control

The plasmid is confirmed by full-length sequencing.

Screening

Antibiotic in E.coli

Ampicillin

Antibiotic in Mammalian cell

Hygromycin

Application

Stable or Transient mammalian expression

Storage & Shipping

Shipping

Each tube contains lyophilized plasmid.

Storage

The lyophilized plasmid can be stored at ambient temperature for three months.

Other Life Science vendor

Natural small molecules manufacturer | Biological Research Reagents Manufacturer

Background Information

The protein encoded by SART3 gene is an RNA-binding nuclear protein that is a tumor-rejection antigen. This antigen possesses tumor epitopes capable of inducing HLA-A24-restricted and tumor-specific cytotoxic T lymphocytes in cancer patients and may be useful for specific immunotherapy. This gene product is found to be an important cellular factor for HIV-1 gene expression and viral replication. It also associates transiently with U6 and U4/U6 snRNPs during the recycling phase of the spliceosome cycle. This encoded protein is thought to be involved in the regulation of mRNA splicing.

References

Liu Y, Timani K, Ou X, Broxmeyer HE, He JJ. C-MYC Controlled TIP110 Protein Expression Regulates OCT4 mRNA Splicing in Human Embryonic Stem Cells. Stem Cells and Development. 2013;22(5):689-694.

Catalog Number:HGG826-UT