Human PTH2 / PTRH2 Gene ORF cDNA clone expression plasmid,C terminal GFP tag

Catalog Number:HGG262-CG

Gene
Species
Human
NCBI Ref Seq
RefSeq ORF Size
540bp
Gene Synonym
BIT1, PTH2, CGI-147, PTRH2
Sequence Description
Identical with the Gene Bank Ref. ID sequence.
Description
Full length Clone DNA of Human peptidyl-tRNA hydrolase 2 Gene ORF cDNA clone expression plasmid,C terminal GFP tag
Plasmid
Promoter
Enhanced CMV mammalian cell promoter
Vector
pCMV3-C-GFPSpark
Restriction Site
Protein Tag
GFPSpark
Tag Sequence
GTGAGCAAGGGC……GAGCTGTACAAG
Sequencing Primers
Forward:T7(TAATACGACTCACTATAGGG) Reverse:BGH(TAGAAGGCACAGTCGAGG)
Quality Control
The plasmid is confirmed by full-length sequencing.
GFPSpark Tag Information
GFPSpark is an improved variant of the green fluorescent protein GFP. It possesses bright green fluorescence (excitation/ emission max = 487 / 508 nm) that is visible earlier than fluorescence of other green fluorescent proteins. GFPSpark is mainly intended for applications where fast appearance of bright fluorescence is crucial. It is specially recommended for cell and organelle labeling and tracking the promoter activity.
Screening
Antibiotic in E.coli
Kanamycin
Antibiotic in Mammalian cell
Hygromycin
Application
Stable or Transient mammalian expression
Storage & Shipping
Shipping
Each tube contains lyophilized plasmid.
Storage
The lyophilized plasmid can be stored at ambient temperature for three months.
Background Information
PTH2, also known as PTRH2, is a mitochondrial protein which belongs to the PTH2 family. PTH 2 is released during apoptosis from the mitochondria to the cytoplasm. The natural substrate for PTH 2 may be peptidyl-tRNAs which drop off the ribosome during protein synthesis. When in the cytoplasm, PTRH2 regulates the function of 2 transcriptional regulators, TLE5 and TLE1, thus promoting caspase-independent cell death.
References
  • Prahalad AK. et al., 2006, Proteomics. 6 (12): 3482-93.
  • Jin L. et al., 2000, J Biol Chem. 275 (35): 27238-44.
  • Boone T. et al., 2000, Nat Struct Biol. 7 (6): 486-91.
  • TOP