Human MLANA Gene ORF cDNA clone expression plasmid,N terminal GFP tag

Gene

Species

Human

NCBI Ref Seq

BC014423

RefSeq ORF Size

357bp

Gene Synonym

MART1, MART-1, MLANA

Sequence Description

Identical with the Gene Bank Ref. ID sequence.

Description

Full length Clone DNA of Human melan-A Gene ORF cDNA clone expression plasmid,N terminal GFP tag

Plasmid

Promoter

Enhanced CMV mammalian cell promoter

Vector

pCMV3-N-GFPSpark

Restriction Site

Protein Tag

GFPSpark

Tag Sequence

GTGAGCAAGGGC……GAGCTGTACAAG

Sequencing Primers

Forward:T7(TAATACGACTCACTATAGGG) Reverse:BGH(TAGAAGGCACAGTCGAGG)

Quality Control

The plasmid is confirmed by full-length sequencing.

GFPSpark Tag Information

GFPSpark is an improved variant of the green fluorescent protein GFP. It possesses bright green fluorescence (excitation/ emission max = 487 / 508 nm) that is visible earlier than fluorescence of other green fluorescent proteins. GFPSpark is mainly intended for applications where fast appearance of bright fluorescence is crucial. It is specially recommended for cell and organelle labeling and tracking the promoter activity.

Screening

Antibiotic in E.coli

Kanamycin

Antibiotic in Mammalian cell

Hygromycin

Application

Stable or Transient mammalian expression

Storage & Shipping

Shipping

Each tube contains lyophilized plasmid.

Storage

The lyophilized plasmid can be stored at ambient temperature for three months.

Other Life Science vendor

Natural small molecules manufacturer | Biological Research Reagents Manufacturer

Background Information

MART-1 is a hydrophobic transmembrane protein without glycosylation sites highly enriched in early melanosomes. MART-1 is necessary for gp100 function, another antigen associated to cutaneous melanom, involved in the regulation of melanosome formation. MART-1 is expressed in skin and retinae melanocytes and in the majority of melanoma tumors, but it is absent from other tissues and tumors. It was isolated thanks to the specific recognition by T lymphocytes of MART-1 derived peptides, specially in the context of the HLA-A0201 haplotype, present in tumor infiltrates from melanoma patients. Thus, MART-1 is immunogenic in humans and has been widely exploited to induce anti-melanoma immunity in patients by means of several vaccination strategies.

References

Barrio MM, Abes R, Colombo M, et al. Human Macrophages and Dendritic Cells Can Equally Present MART-1 Antigen to CD8+ T Cells after Phagocytosis of Gamma-Irradiated Melanoma Cells. Chatenoud L, ed. PLoS ONE. 2012;7(7):e40311.

Catalog Number:HGE872-NG