Human CTAG2 transcript variant 2 Gene ORF cDNA clone expression plasmid,C terminal His tag

Gene

Species

Human

NCBI Ref Seq

NM_020994.4

RefSeq ORF Size

633bp

Gene Synonym

CT2; ESO2; CAMEL; CT6.2; CT6.2a; CT6.2b; LAGE-1; LAGE2B

Sequence Description

Identical with the Gene Bank Ref. ID sequence.

Description

Full length Clone DNA of Human cancer/testis antigen 2 Gene ORF cDNA clone expression plasmid,C terminal His tag

Plasmid

Promoter

Enhanced CMV mammalian cell promoter

Vector

pCMV3-C-His

Restriction Site

Protein Tag

His

Tag Sequence

CACCATCACCACCATCATCACCACCATCAC

Sequencing Primers

Forward:T7(TAATACGACTCACTATAGGG) Reverse:BGH(TAGAAGGCACAGTCGAGG)

Quality Control

The plasmid is confirmed by full-length sequencing.

His Tag Information

A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein.

Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokarfyotic expression systems.

Screening

Antibiotic in E.coli

Kanamycin

Antibiotic in Mammalian cell

Hygromycin

Application

Stable or Transient mammalian expression

Storage & Shipping

Shipping

Each tube contains lyophilized plasmid.

Storage

The lyophilized plasmid can be stored at ambient temperature for three months.

Other Life Science vendor

Natural small molecules manufacturer | Biological Research Reagents Manufacturer

Background Information

CAMEL (109 aa) is a tumor-specific protein translated from the LAGE-1 gene in an alternative open reading frame (ORF) starting at a second ATG start codon located 40 bp downstream of the first ATG start site. LAGE-1 shows 94% homology to tumor Ag NY-ESO-1 and both genes are located on chromosome Xp28. The LAGE-1 and NY-ESO-1 genes are frequently coexpressed in a wide variety of tumors, like melanoma, breast carcinoma, prostate and bladder cancers, but silent in normal tissues except for testis.

References

Slager E H, van der Minne C E, Krüse M, et al. Identification of multiple HLA-DR-restricted epitopes of the tumor-associated antigen CAMEL by CD4+ Th1/Th2 lymphocytes[J]. The Journal of Immunology, 2004, 172(8): 5095-5102.

Catalog Number:HGB892-CH