Human COL5A2 Gene ORF cDNA clone expression plasmid,without any tag

Gene

Species

Human

NCBI Ref Seq

NM_000393.3

RefSeq ORF Size

4500bp

Gene Synonym

COL5A2

Sequence Description

Identical with the Gene Bank Ref. ID sequence.

Description

Full length Clone DNA of Human collagen, type V, alpha 2 Gene ORF cDNA clone expression plasmid,without any tag

Plasmid

Promoter

Enhanced CMV mammalian cell promoter

Vector

pCMV3-untagged

Restriction Site

Protein Tag

Tag Sequence

Sequencing Primers

Forward:T7(TAATACGACTCACTATAGGG) Reverse:BGH(TAGAAGGCACAGTCGAGG)

Quality Control

The plasmid is confirmed by full-length sequencing.

Screening

Antibiotic in E.coli

Ampicillin

Antibiotic in Mammalian cell

Hygromycin

Application

Stable or Transient mammalian expression

Storage & Shipping

Shipping

Each tube contains lyophilized plasmid.

Storage

The lyophilized plasmid can be stored at ambient temperature for three months.

Other Life Science vendor

Natural small molecules manufacturer | Biological Research Reagents Manufacturer

Background Information

COL5A2 is a component of type V collagen. It is known as the pro-alpha2(V) chain. COL5A2, together with two pro-alpha1(V) chains can form type V procollagen. These triple-stranded, rope-like procollagen molecules arrange themselves into long, thin fibrils that cross-link to one another in the spaces around cells. The cross-links result in the formation of very strong, mature type V collagen fibers. Type V collagen can be detected in tissues containing type I collagen and appears to regulate the assembly of heterotypic fibers composed of both type I and type V collagen.

References

Greenspan DS. et al., 1992, Genomics. 12 (4): 836-7.

Mann K. 1992, Biol Chem Hoppe-Seyler. 373 (2): 69-75.

Greenspan DS. et al., 1992, Gene Expr. 1 (1): 29-39.

Catalog Number:HGB738-UT