Hepatitis C virus (HCV-1a) E2 Gene ORF cDNA clone expression plasmid,N terminal His tag

Catalog Number:VGD533-NH

Gene
Species
HCV
NCBI Ref Seq
RefSeq ORF Size
1140 bp
Gene Synonym
HCV-E2
Sequence Description
Identical with the Gene Bank Ref. ID sequence.
Description
Full length Clone DNA of HCV Hepatitis C virus (HCV) (serotype 1a, isolate H77) E2 Gene ORF cDNA clone expression plasmid,N terminal His tag
Plasmid
Promoter
Enhanced CMV mammalian cell promoter
Vector
pCMV3-N-His
Restriction Site
KpnI + XbaI(6kb+1.14kb)
Protein Tag
His
Tag Sequence
CACCATCACCACCATCATCACCACCATCAC
Sequencing Primers
Forward:T7(TAATACGACTCACTATAGGG) Reverse:BGH(TAGAAGGCACAGTCGAGG)
Quality Control
The plasmid is confirmed by full-length sequencing.
His Tag Information

A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein.

Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokarfyotic expression systems.

Screening
Antibiotic in E.coli
Kanamycin
Antibiotic in Mammalian cell
Hygromycin
Application
Stable or Transient mammalian expression
Storage & Shipping
Shipping
Each tube contains lyophilized plasmid.
Storage
The lyophilized plasmid can be stored at ambient temperature for three months.
Background Information
References
TOP