Human Smad2 Gene ORF cDNA clone expression plasmid,without any tag

Gene

Species

Human

NCBI Ref Seq

NM_001003652.2

RefSeq ORF Size

1404bp

Gene Synonym

Smad2, JV18, MADH2, MADR2, JV18-1, hMAD-2, hSMAD2

Sequence Description

Identical with the Gene Bank Ref. ID sequence.

Description

Full length Clone DNA of Human SMAD family member 2 (SMAD2), transcript variant 2 Gene ORF cDNA clone expression plasmid,without any tag

Plasmid

Promoter

Enhanced CMV mammalian cell promoter

Vector

pCMV3-untagged

Restriction Site

Protein Tag

Tag Sequence

Sequencing Primers

Forward:T7(TAATACGACTCACTATAGGG) Reverse:BGH(TAGAAGGCACAGTCGAGG)

Quality Control

The plasmid is confirmed by full-length sequencing.

Screening

Antibiotic in E.coli

Ampicillin

Antibiotic in Mammalian cell

Hygromycin

Application

Stable or Transient mammalian expression

Storage & Shipping

Shipping

Each tube contains lyophilized plasmid.

Storage

The lyophilized plasmid can be stored at ambient temperature for three months.

Other Life Science vendor

Natural small molecules manufacturer | Biological Research Reagents Manufacturer

Background Information

SMAD2 is a member of the SMAD family. Members of this family mediate signal transduction by the TGF-beta/activin/BMP-2/4 cytokine superfamily from receptor Ser/Thr protein kinases at the cell surface to the nucleus. SMAD2 mediates the signal of the TGF-beta, and therefore regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. SMAD2 is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. SMAD2 is the downstream signal transducers of TGF-beta-1 in human dental pulp cells. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors. Phosphorylated SMAD2 is able to form a complex with SMAD4 or SARA. These complexes accumulate in the cell nucleus, where they are directly participating in the regulation of gene expression.

References

Feng. et al., 2002, Mol Cell. 9 (1): 133-43.

Zhu Y. et al., 1997, J Biol Chem. 272 (15): 10035-40.

Zi Z. et al., 2012, FEBS Lett. 586 (14): 1921-8.

Catalog Number:RGH211-UT